Comparative Safety Evaluation of Boma and Chemical Immobilization Techniques Applied for Translocation of Spotted Deer (Axis axis)

Boma and Chemical Immobilization for Translocating Spotted Deer


  • Bushra Nisar Khan Conservation Biology Lab, Institute of Zoology, University of Punjab, Lahore, Pakistan
  • Muhammad Rizwan Khan Safari Zoo, Punjab Wildlife and Parks Department, Lahore, Pakistan
  • Muhammad Azhar Safari Zoo, Punjab Wildlife and Parks Department, Lahore, Pakistan
  • Rida Fatima University of Veterinary and Animal Sciences, Lahore, Pakistan
  • Abida Mushtaque Center of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
  • Muhammad Talha Mukhtar Conservation Biology Lab, Institute of Zoology, University of Punjab, Lahore, Pakistan
  • Aneela Anwar Department of Chemistry, University of Engineering and Technology, Lahore, Pakistan



Translocation, Boma, Spotted Deer, Capture Myopathy, Chemical Restraint


The translocation of wild ungulates poses significant stress to the animal species being transported, necessitating careful consideration. Chemical restraint helps to reduce the stress levels significantly by minimizing the mobility of the animals. Capture myopathy, a leading cause of mortality during chemical immobilization, is a critical concern. However, the Boma method offers a promising alternative to the conventionally used translocation methods. Objective: To compare the survival outcomes of the spotted deer captured and transported using both chemical restraint and the Boma method, therefore assessing their respective effectiveness. Methods: In the study, we used 22 spotted deer for chemical restraint method and 30 spotted deer were captured through Boma method and both of techniques were assessed, how capture methods influenced the fatality rate of the highly sensitive spotted deer which were being trans-located from Safari Zoo, Lahore to other captive sites of Punjab Pakistan. Results: The exceptional record of zero mortality in the Boma procedure was seen as compared to the chemical immobilization in which 31% of the total shifted animals were expired. The pathognomic white streaked/pale muscles in necropsy findings of dead animals later on confirmed the dead due to capture myopathy. Conclusions: The study established the finding that Boma technique is safer technique for trans-location of ungulates especially spotted deer as compared to the chemical immobilization. 


Talukder ABI, Islam MR, Faruk A, Akhter F. Effective management strategies for spotted deer at the national zoo in Bangladesh. American journal of Zoology. 2023; 6(3): 46-52.

Spraker TR. Stress and capture myopathy in artiodactylids. Zoo and Wild Animal Medicine. 1993: 481-8.

Manoa DO and Mwaura F. Predator-proof bomas as a tool in mitigating human-predator conflict in loitokitok sub-county amboseli region of Kenya. Natural Resources. 2016; 7(01): 28. doi: 10.4236/nr.2016.71003.

Kruger M. Capture and boma stress responses in the white rhinoceros (Ceratotherium simum): University of the Witwatersrand, Faculty of Science; 2017.

Murray S, Monfort SL, Ware L, McShea WJ, Bush M. Anesthesia in female white-tailed deer using Telazol® and Xylazine. Journal of Wildlife Diseases. 2000 Oct; 36(4): 670-5. doi: 10.7589/0090-3558-36.4.670.

Mathieu A, Caulkett N, Stent PM, Schwantje HM. Capture of free-ranging mule deer (Odocoileus hemionus) with a combination of medetomidine, azaperone, and alfaxalone. Journal of Wildlife Diseases. 2017 Apr; 53(2): 296-303. doi: 10.7589/2016-09-210.

Knox CM, Zeller D, Hattingh J. Comparison of two methods for the capture of Boma-confined impala. South African Journal of Wildlife Research-24-month Delayed Open Access. 1993 Jan; 23(1): 1-5.

Hofmeyr J and Lenssen J. The capture and care of eland Taurotragus oryx oryx (Pallas) using the Boma method. Madoqua. 1975 Jan; 1975(2): 25-33.

Sankar K, Pabla H, Patil C, Nigam P, Qureshi Q, Navaneethan B, et al. Home range, habitat use and food habits of re-introduced gaur (Bos Gaurus Gaurus) in Bandhavgarh Tiger Reserve, Central India. Tropical Conservation Science. 2013 Mar; 6(1): 50-69. doi: 10.1177/194008291300600108.

Augustine DJ, Veblen KE, Goheen JR, Riginos C, Young TP. Pathways for positive cattle-wildlife interactions in semiarid rangelands. Smithsonian Contributions to Zoology. 2011 Jan; 632: 55-71. doi: 10.5479/si.00810282.632.55.

Citino SB, Bush M, Grobler D, Lance W. Anesthesia of Boma-captured Lichtenstein's hartebeest (Sigmoceros lichtensteinii) with a combination of thiafentanil, medetomidine, and ketamine. Journal of Wildlife Diseases. 2002 Apr; 38(2): 457-62. doi: 10.7589/0090-3558-38.2.457.

Izwan A, Snelling EP, Seymour RS, Meyer LC, Fuller A, Haw A, Mitchell D, Farrell AP, Costello MA, Maloney SK et al. Ameliorating the adverse cardiorespiratory effects of chemical immobilization by inducing general anesthesia in sheep and goats: Implications for physiological studies of large wild mammals. Journal of Comparative Physiology B. 2018 Nov; 188(6): 991-1003. doi: 10.1007/s00360-018-1184-z.

Marco I and Lavin S. Effect of the method of capture on the hematology and blood chemistry of red deer (Cervus elaphus). Research in Veterinary Science. 1999 Apr; 66(2): 81-4. doi: 10.1053/rvsc.1998.0248.

Paterson JM, Caulkett NA, Woodbury MR. Physiologic effects of nasal oxygen or medical air administered prior to and during carfentanil-xylazine anesthesia in North American elk (Cervus canadensis manitobensis). Journal of Zoo and Wildlife Medicine. 2009 Mar; 40(1): 39-50. doi: 10.1638/2007-0107.1

Avni-Magen N, Zafrir B, King R, Bdolah-Abram T, Shilo-Benjamini Y. Immobilization of captive Persian fallow deer (Dama dama mesopotamica) using medetomidine-ketamine or medetomidine-midazolam. Veterinary Anaesthesia and Analgesia. 2019 Sep; 46(5): 662-6. doi: 10.1016/j.vaa.2019.06.003.

Monfort SL, Brown JL, Wildt DE. Episodic and seasonal rhythms of cortisol secretion in male Eld's deer (Cervus eldi thamin). Journal of Endocrinology. 1993 Jul; 138(1): 41-9. doi: 10.1677/joe.0.1380041.

Klein L, Raphael BL, Kalk P, Cook RA, editors. Immobilization of Eld's Deer (Cervus eldi): Medetomidine-Ketamine Versus Carfentanil. Annual Conference-American Association of Zoo Veterinarians; 1996: American Association of Zoo Veterinarians. 1996: 376-81.

Cromwell JA, Warren RJ, Henderson DW. Live-capture and small-scale relocation of urban deer on Hilton Head Island, South Carolina. Wildlife Society Bulletin. 1999 Dec: 1025-31.

Robinson S, Milner‐Gulland E, Grachev Y, Salemgareyev A, Orynbayev M, Lushchekina A, et al. Opportunistic bacteria and mass mortality in ungulates: lessons from an extreme event. Ecosphere. 2019 Jun; 10(6): e02671. doi: 10.1002/ecs2.2671.

Huber N, Vetter SG, Evans AL, Kjellander P, Küker S, Bergvall UA, et al. Quantifying capture stress in free ranging European roe deer (Capreolus capreolus). BMC Veterinary Research. 2017 Dec; 13: 1-9. doi: 10.1186/s12917-017-1045-0.

Talbot LM. Ecological aspects of aid programs in East Africa, with particular reference to rangelands. Bulletins from the Ecological Research Committee. 1971 Jan (13): 21-51.

Pon K, Caulkett N, Woodbury M. Efficacy and safety of a medetomidine-azaperone-alfaxalone combination in captive white-tailed deer (Odocoileus virginianus). Journal of Zoo and Wildlife Medicine. 2016 Mar; 47(1): 29-37. doi: 10.1638/2015-0121.1.

Schwartz JA, Warren RJ, Henderson DW, Osborn DA, Kesler DJ. Captive and field tests of a method for immobilization and euthanasia of urban deer. Wildlife Society Bulletin. 1997 Jul: 532-41.



DOI: 10.54393/mjz.v5i01.95
Published: 2024-03-31

How to Cite

Khan, B. N., Khan, M. R., Azhar, M., Fatima, R., Mushtaque, A., Mukhtar, M. T., & Anwar, A. (2024). Comparative Safety Evaluation of Boma and Chemical Immobilization Techniques Applied for Translocation of Spotted Deer (Axis axis) : Boma and Chemical Immobilization for Translocating Spotted Deer . MARKHOR (The Journal of Zoology), 5(01), 40–44.


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