Antibiotic Susceptibility and Resistance of Clinical Isolates against Various Antibiotics

Antibiotic Susceptibility and Resistance


  • Ansar Abbas Virtual University, Lahore, Pakistan
  • Sadia Sarwar Lahore College for Women University, Lahore, Pakistan



Antibiotic Susceptibility, Resistance, Streptococcus pyogenes, Staphylococcus aureus


Antibiotics are essential for treating bacterial infections, but their overuse and misuse have led to the development of antibiotic resistance. Objective: To evaluate the antibiotic susceptibility and resistance of clinical isolates against various antibiotics. Methods: A total of 1000 clinical isolates were collected from patients at the Fatima Memorial Hospital in Lahore, Pakistan. Identification of bacteria was performed using conventional culture and biochemical tests. Antibiotic susceptibility was assessed using the Kirby-Bauer disc diffusion method with 26 commercial antibiotic discs, including amikacin, amoxycillin, ampicillin, and others. Results: The study found that the most common Gram-positive isolates were Staphylococcus aureus (40.0%) and Streptococcus pyogenes (5.0%). The most effective antibiotics against these isolates were amikacin, cefotaxime, and meropenem. In contrast, high levels of resistance were observed against commonly prescribed antibiotics, including amoxicillin, ampicillin, and cefixime. Conclusion: These findings highlight the need to promote appropriate antibiotic use and to monitor antibiotic resistance patterns to combat the rise of antibiotic-resistant infections


Ventola CL. The antibiotic resistance crisis: part 1: causes and threats. Pharmacy and Therapeutics. 2015 Apr; 40(4): 277-283.

Livermore DM. Fourteen years in resistance. International Journal of Antimicrobial Agents. 2012 Apr; 39(4): 283-94. doi: 10.1016/j.ijantimicag.2011.12.012

Sahoo KC. Antibiotic Resistance and Environmental Factors. 2012. Available at:

Andersson DI and Hughes D. Antibiotic resistance and its cost: is it possible to reverse resistance?. Nature Reviews Microbiology. 2010 Apr; 8(4): 260-71. doi: 10.1038/nrmicro2319

Mehta A, Rosenthal VD, Mehta Y, Chakravarthy M, Todi SK, Sen N, et al. Device-associated nosocomial infection rates in intensive care units of seven Indian cities. Findings of the International Nosocomial Infection Control Consortium (INICC). Journal of Hospital Infection. 2007 Oct; 67(2): 168-74. doi: 10.1016/j.jhin.2007.07.008

Khan RA, Rahman AU, Ahmad A, Jaseem M, Jabbar A, Khan SA, et al. Prevalence and antibiotic susceptibility profile of methicillin-resistant Staphylococcus aureus (MRSA) isolated from different clinical samples in district Peshawar. Journal of Applied Environmental Biological Science. 2014 Nov; 4(8S): 40-6.

Shenoy S, Baliga S, Saldanha DR, Prashanth HV. Antibiotic sensitivity patterns of Pseudomonas aeruginosa strains isolated from various clinical specimens. Indian Journal of Medical Sciences. 2002 Sep; 56(9): 427-30.

Barakoti A, Guragain A, Adhikari RP, Amatya R. Profile and antimicrobial susceptibility pattern of aerobic bacterial isolates from pus/wound swab samples in a tertiary care hospital, Kathmandu. Nepal Medical College Journal. 2017 Jan; 19(4):179-83.

Javeed I, Hafeez R, Anwar MS. Antibiotic susceptibility pattern of bacterial isolates from patients admitted to a tertiary care hospital in Lahore. Biomedica. 2011 Jan; 27(2): 19-23.

Hasan B, Perveen K, Olsen B, Zahra R. Emergence of carbapenem-resistant Acinetobacter baumannii in hospitals in Pakistan. Journal of Medical Microbiology. 2014 Jan; 63(1): 50-5. doi: 10.1099/jmm.0.063925-0

Pathi B, Mishra SN, Panigrahi K, Poddar N, Lenka P, Mallick B, et al. Prevalence and antibiogram pattern of Pseudomonas aeruginosa in a tertiary care hospital from Odisha, India. Transworld Medical Journal. 2013 Jan; 1(3):77-80.

Rangaiahagari A, Uwizeyimana JP, Nyirabanzi J, Ngoga E, Wane J. Antibiotic sensitivity patterns of Enterobacteriaceae isolated at King Faisal Hospital, Kigali: A three years study. Rwanda Medical Journal. 2013 Mar; 70: 11-4.

Gums JG. Magnesium in cardiovascular and other disorders. American Journal of Health-System Pharmacy. 2004 Aug; 61(15): 1569–76. doi: 10.1093/ajhp/61.15.1569

Hsueh PR, Chen WH, Luh KT. Relationships between antimicrobial use and antimicrobial resistance in Gram-negative bacteria causing nosocomial infections from 1991–2003 at a university hospital in Taiwan. International Journal of Antimicrobial Agents. 2005 Dec; 26(6): 463-72. doi: 10.1016/j.ijantimicag.2005.08.016

Kaleem F, Usman J, Hassan A, Omair M, Khalid A, Uddin R. Sensitivity pattern of methicillin resistant Staphylococcus aureus isolatedfrom patients admitted in a tertiary care hospital of Pakistan. Iranian Journal of Microbiology. 2010 Sep; 2(3): 141-3.

Arayne MS, Sultana NA, Khanum FO, Ali MA. Antibacterial studies of cefixime copper, zinc and cadmium complexes. Pakistan Journal of Pharmaceutical Sciences. 2002 Jan; 15: 1-8.

Perveen I, Majid A, Knawal S, Naz I, Sehar S, Ahmed S, et al. Prevalence and antimicrobial susceptibility pattern of methicillin-resistant Staphylococcus aureus and coagulase-negative Staphylococci in Rawalpindi, Pakistan. British Journal of Medicine and Medical Research. 2013 Jan; 3(1): 198-209. doi: 10.9734/BJMMR/2013/2109

Baddour MM, Abuelkheir MM, Fatani AJ. Trends in antibiotic susceptibility patterns and epidemiology of MRSA isolates from several hospitals in Riyadh, Saudi Arabia. Annals of Clinical Microbiology and Antimicrobials. 2006 Dec; 5(1): 1-1.

Vanitha RN, Kannan G, Venkata NM, Vishwakanth D, Nagesh VR, Yogitha M, et al. A retrospective study on blood stream infections and antibiotic susceptibility patterns in a tertiary care teaching hospital. International Journal of Pharmacy and Pharmaceutical Sciences. 2012; 4(1): 543-8.

Shrestha S, Shrestha NC, Singh SD, Shrestha RP, Kayestha S, Shrestha M, et al. Bacterial isolates and its antibiotic susceptibility pattern in NICU. Kathmandu University Medical Journal. 2014 Sep; 11(1): 66-70. doi: 10.3126/kumj.v11i1.11030



DOI: 10.54393/mjz.v3i02.57
Published: 2022-12-31

How to Cite

Abbas, A. ., & Sarwar, S. . (2022). Antibiotic Susceptibility and Resistance of Clinical Isolates against Various Antibiotics: Antibiotic Susceptibility and Resistance. MARKHOR (The Journal of Zoology), 3(02), 29–32.



Original Article