Antibiotic Susceptibility and Resistance of Clinical Isolates against Various Antibiotics
Antibiotic Susceptibility and Resistance
Keywords:Antibiotic Susceptibility, Resistance, Streptococcus pyogenes, Staphylococcus aureus
Antibiotics are essential for treating bacterial infections, but their overuse and misuse have led to the development of antibiotic resistance. Objective: To evaluate the antibiotic susceptibility and resistance of clinical isolates against various antibiotics. Methods: A total of 1000 clinical isolates were collected from patients at the Fatima Memorial Hospital in Lahore, Pakistan. Identification of bacteria was performed using conventional culture and biochemical tests. Antibiotic susceptibility was assessed using the Kirby-Bauer disc diffusion method with 26 commercial antibiotic discs, including amikacin, amoxycillin, ampicillin, and others. Results: The study found that the most common Gram-positive isolates were Staphylococcus aureus (40.0%) and Streptococcus pyogenes (5.0%). The most effective antibiotics against these isolates were amikacin, cefotaxime, and meropenem. In contrast, high levels of resistance were observed against commonly prescribed antibiotics, including amoxicillin, ampicillin, and cefixime. Conclusion: These findings highlight the need to promote appropriate antibiotic use and to monitor antibiotic resistance patterns to combat the rise of antibiotic-resistant infections
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